1x first strand buffer (Thermo Fisher)
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Thermo Fisher
1x first strand buffer
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+first+strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+first+strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x first strand buffer - by Bioz Stars,
2026-10
95/100 stars
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other:Article Title: Article Snippet: Briefly, RNA was isolated as described above, residual genomic DNA was removed via a DNase treatment according to the manufacturer’s protocol ( Quantitative RT-PCR:Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells. Article Snippet: .. RT-qPCR reaction mix included: Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells Article Snippet: .. RT-qPCR reaction mix included: Virus:Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells. Article Snippet: .. RT-qPCR reaction mix included: Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells Article Snippet: .. RT-qPCR reaction mix included: Reverse Transcription:Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells. Article Snippet: .. RT-qPCR reaction mix included: Article Title: High glucose promotes cisplatin chemoresistance in MDA-MB-231 breast cancer derived cells through changes in gene expression and multiple signaling pathways Article Snippet: Total RNA (1 μg) was reverse-transcribed using the SuperScriptTM III First-Strand Synthesis System (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol (50 ̊C for 60 min, followed by enzyme inactivation at 70 ̊C for 15 min). .. Alternatively, when individual components were used, reverse transcription was performed in a 20 μl reaction mixture containing 200 U M-MLV reverse transcriptase, Article Title: High glucose promotes cisplatin chemoresistance in MDA‑MB‑231 breast cancer derived cells through changes in gene expression and multiple signaling pathways Article Snippet: Total RNA (1 μg) was reverse‐transcribed using the SuperScriptTM III First‐Strand Synthesis System (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol (50 ̊C for 60 min, followed by enzyme inactivation at 70 ̊C for 15 min). .. Alternatively, when individual components were used, reverse transcription was performed in a 20 μl reaction mixture containing 200 U M‐MLV reverse transcriptase, Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect Article Snippet: .. To this initial mixture, we added a 25 μl mixture of working concentrations of Article Title: Late-onset epi-cblC methylmalonic aciduria with tissue-variable MMACHC promoter methylation due to a stop retained PRDX1 variant. Article Snippet: Reverse transcription The cDNA was prepared from 1 μg of RNA annealed with 50 pmols of random hexamers (Invitrogen) after denaturation at 65 °C for 5 min and rapid cooling on ice. .. The RNA was reversetranscribed using 200 U of SuperScript III Reverse Transcriptase (Invitrogen) in the presence of Article Title: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells Article Snippet: .. RT-qPCR reaction mix included: Article Title: FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners Article Snippet: .. Then, Incubation:Article Title: High glucose promotes cisplatin chemoresistance in MDA-MB-231 breast cancer derived cells through changes in gene expression and multiple signaling pathways Article Snippet: Total RNA (1 μg) was reverse-transcribed using the SuperScriptTM III First-Strand Synthesis System (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol (50 ̊C for 60 min, followed by enzyme inactivation at 70 ̊C for 15 min). .. Alternatively, when individual components were used, reverse transcription was performed in a 20 μl reaction mixture containing 200 U M-MLV reverse transcriptase, Article Title: High glucose promotes cisplatin chemoresistance in MDA‑MB‑231 breast cancer derived cells through changes in gene expression and multiple signaling pathways Article Snippet: Total RNA (1 μg) was reverse‐transcribed using the SuperScriptTM III First‐Strand Synthesis System (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol (50 ̊C for 60 min, followed by enzyme inactivation at 70 ̊C for 15 min). .. Alternatively, when individual components were used, reverse transcription was performed in a 20 μl reaction mixture containing 200 U M‐MLV reverse transcriptase, Sequencing:Article Title: FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners Article Snippet: .. Then, Modification:Article Title: FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners Article Snippet: .. Then, |